NANS Protein (AA 1-359) (His tag)

Produktnummer ABIN667265

Produktdetails

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-359

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses NANS Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: NANS, 1-359aa, Human, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Lagerung: 4 °C

Antigendetails

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Andere Bezeichnung: NANS (NANS Produkte)

Synonyme: MGC84329 Protein, SAS Protein, N-acetylneuraminate synthase L homeolog Protein, N-acetylneuraminate synthase Protein, nans.L Protein, NANS Protein, Nans Protein

Hintergrund: NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Sialic acid synthase, SAS, N acetylneuraminate synthase. NCBI no.: NP_061819

Molekulargewicht: 42.4 kDa (379aa) confirmed by MALDI-TOF