MSRB1 Protein (AA 1-116) (His tag)

Produktnummer ABIN667227

Produktdetails

Target: MSRB1 (SEPX1) (Selenoprotein X, 1 (SEPX1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-116

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MSRB1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: SEPX1, 1-116aa, Human, His tag, E.coli

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2mM EDTA, 10% Glycerol

Lagerung: 4 °C

Antigendetails

Target: MSRB1 (SEPX1) (Selenoprotein X, 1 (SEPX1))

Andere Bezeichnung: SEPX1 (SEPX1 Produkte)

Synonyme: MsrB Protein, MsrB1-A Protein, SePR Protein, SelX-A Protein, cb687 Protein, selx Protein, sepx1 Protein, sepx1a Protein, wu:fa03e09 Protein, SEPX1 Protein, SELR Protein, SELX Protein, SepR Protein, D17Wsu82e Protein, SelR Protein, Sepr Protein, Sepx1 Protein, selX Protein, MSRB1 Protein, DKFZp469G2418 Protein, methionine sulfoxide reductase B1a Protein, methionine sulfoxide reductase B1 Protein, selenoprotein X, 1 Protein, msrb1a Protein, MSRB1 Protein, msrb1 Protein, Sepx1 Protein, Msrb1 Protein

Hintergrund: SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases(MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/U) element is located immediately following the UGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Selenoprotein X, HSPC270, MSRB1, SELR, SELX, MSRB1, Methionine-R-sulfoxide reductase B1, U95C. NCBI no.: NP_057416

Molekulargewicht: 14.8 kDa(136aa), confirmed by MALDI-TOF