Hba-x Protein (AA 1-142) (His tag)

Produktnummer ABIN6388171

Produktdetails

Target: Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-142

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses Hba-x Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH

Reinheit: > 85 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1ug of protein (determined by LAL method)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In 50  mM Tris-HCl (  pH 7.5) containing 30 % glycerol, 0.5M NaCl, 2  mM DTT, 0.1  mM PMSF

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Hba-x (HBA-X) (Hemoglobin X, alpha-Like Embryonic Chain in Hba Complex (HBA-X))

Andere Bezeichnung: Hba-x

Synonyme: AI450015 Protein, Hbz Protein, hemoglobin X, alpha-like embryonic chain in Hba complex Protein, Hba-x Protein

Hintergrund: RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 60.2kDa (533aa) 70-100kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_003152

UniProt: P23443