GNMT Protein (AA 1-295) (His tag)
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- Target Alle GNMT Proteine anzeigen
- GNMT (Glycine N-Methyltransferase (GNMT))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-295
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses GNMT Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
- Reinheit
- > 95 % by SDS - PAGE
- Endotoxin-Niveau
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
- Top Product
- Discover our top product GNMT Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GNMT (Glycine N-Methyltransferase (GNMT))
- Andere Bezeichnung
- GNMT (GNMT Produkte)
- Synonyme
- glycine N-methyltransferase Protein, CpipJ_CPIJ015956 Protein, Gnmt Protein, GNMT Protein
- Hintergrund
- Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 34.9 kDa (315aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_061833
- UniProt
- Q14749
- Pathways
- Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process
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