ECHS1 Protein (AA 28-290) (His tag)
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- Target Alle ECHS1 Proteine anzeigen
- ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 28-290
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses ECHS1 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.
- Top Product
- Discover our top product ECHS1 Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 100 mM NaCl
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))
- Andere Bezeichnung
- ECHS1 (ECHS1 Produkte)
- Synonyme
- SCEH Protein, cOR6not Protein, fj55e05 Protein, si:zc217g15.1 Protein, wu:fj55e05 Protein, C80529 Protein, enoyl-CoA hydratase, short chain, 1, mitochondrial L homeolog Protein, enoyl-CoA hydratase, short chain 1 Protein, enoyl CoA hydratase, short chain, 1, mitochondrial Protein, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial Protein, echs1.L Protein, ECHS1 Protein, Echs1 Protein, echs1 Protein
- Hintergrund
- Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 30.6 kDa (284aa), confirmed by MALDI-TOF
- Pathways
- Monocarboxylic Acid Catabolic Process
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