PGP Protein (AA 1-321) (His tag)
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- Target Alle PGP Proteine anzeigen
- PGP (Phosphoglycolate Phosphatase (PGP))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-321
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses PGP Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product PGP Protein
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Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (His tag)
PGP Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (Strep Tag)Crystallography grade PGP Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (Strep Tag)Crystallography grade PGP Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycolate Phosphatase (PGP) protein (Myc-DYKDDDDK Tag)PGP Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PGP (Phosphoglycolate Phosphatase (PGP))
- Andere Bezeichnung
- PGP (PGP Produkte)
- Synonyme
- RGD1307773 Protein, cb241 Protein, sb:cb241 Protein, wu:fb92c03 Protein, wu:fi40b02 Protein, zgc:56011 Protein, 1700012G19Rik Protein, AI481330 Protein, phosphoglycolate phosphatase Protein, Pgp Protein, PGP Protein, pgp Protein
- Hintergrund
- Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 36.5 kDa (345aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001035830
- UniProt
- A6NDG6
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