PHPT1 Protein (AA 1-125) (His tag)
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- Target Alle PHPT1 Proteine anzeigen
- PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-125
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses PHPT1 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH7.5 at 37C.
- Top Product
- Discover our top product PHPT1 Protein
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phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125) protein (GST tag)
PHPT1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA, PAA
phosphohistidine Phosphatase 1 (PHPT1) (AA 2-125) protein (His tag)Crystallography grade PHPT1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125), (full length) protein (GST tag)PHPT1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125), (full length) protein (His tag)PHPT1 Spezies: Human Wirt: Hefe Recombinant > 90 % SDS
phosphohistidine Phosphatase 1 (PHPT1) proteinPHPT1 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
phosphohistidine Phosphatase 1 (PHPT1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PHPT1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 2 mM DTT, 10 % glycerol
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
- Andere Bezeichnung
- PHPT1 (PHPT1 Produkte)
- Synonyme
- wu:fb50e08 Protein, zgc:101086 Protein, MGC131298 Protein, PHP14 Protein, CGI-202 Protein, HSPC141 Protein, RP11-216L13.10 Protein, bA216L13.10 Protein, 1700008C22Rik Protein, Php14 Protein, phosphohistidine phosphatase 1 Protein, phosphohistidine phosphatase 1 L homeolog Protein, 14 kDa phosphohistidine phosphatase-like Protein, LOC424401 Protein, phpt1 Protein, PHPT1 Protein, phpt1.L Protein, LOC100511240 Protein, Phpt1 Protein
- Hintergrund
- PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 15.9 kDa (145aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_054891
- UniProt
- Q9NRX4
- Pathways
- Positive Regulation of Peptide Hormone Secretion
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