PPIF Protein (AA 30-206) (His tag)

Produktnummer ABIN6387945

Produktdetails

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 30-206

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PPIF Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS

Reinheit: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In Phosphate buffered saline (  pH 7.4) containing 10 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Andere Bezeichnung: Ppif (PPIF Produkte)

Synonyme: CYP3 Protein, CyP-M Protein, Cyp-D Protein, CypD Protein, CyP-D Protein, ppif Protein, wu:fd61c06 Protein, zgc:101753 Protein, AW457192 Protein, CyP-F Protein, PPIase Protein, Cyp-40 Protein, cypf Protein, peptidylprolyl isomerase F Protein, peptidylprolyl isomerase Fa Protein, peptidylprolyl isomerase F (cyclophilin F) Protein, peptidylprolyl isomerase D Protein, peptidylprolyl isomerase F L homeolog Protein, PPIF Protein, ppifa Protein, Ppif Protein, Ppid Protein, ppif Protein, ppif.L Protein

Hintergrund: Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 21.2Da (200aa) confirmed by MALDI-TOF

NCBI Accession: NP_758443

UniProt: P29117

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity