CST6 Protein (AA 29-149) (His tag)
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- Target Alle CST6 Proteine anzeigen
- CST6 (Cystatin E/M (CST6))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 29-149
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses CST6 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM
- Reinheit
- > 90 % by SDS - PAGE
- Biological Activity Comment
- The IC50 value is < 10nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
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Cystatin E/M (CST6) (AA 61-149) protein (GST tag)
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Cystatin E/M (CST6) (AA 29-149) protein (GST tag)CST6 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin E/M (CST6) protein (Myc-DYKDDDDK Tag)CST6 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer( pH 8.0) containing 10 % glycerol, 1 mM DTT, 0.1M NaCl.
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CST6 (Cystatin E/M (CST6))
- Andere Bezeichnung
- CST6 (CST6 Produkte)
- Synonyme
- CST6 Protein, 1110017E11Rik Protein, N28197 Protein, ichq Protein, cystatin E/M Protein, CST6 Protein, Cst6 Protein
- Hintergrund
- CST6 is a member of the type 2 cystatin family. Some of the members are active cysteine protease inhibitors, while cystatin E/M moderate the inhibition of cathepsin B but is not active against cathepsin C. CST6 is secretable proteins that influence osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and the response to systemic inflammation. Recombinant human CST6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 15.9 kDa (142aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_001314
- UniProt
- Q15828
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