CSTA Protein (AA 1-98) (His tag)
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- Target Alle CSTA Proteine anzeigen
- CSTA (Cystatin A (Stefin A) (CSTA))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-98
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses CSTA Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- The IC50 value is < 1.0nM. The inhibitory function of Cystatin A on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
- Top Product
- Discover our top product CSTA Protein
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)
CSTA Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin A (Stefin A) (CSTA) (AA 1-97) protein (Strep Tag)Crystallography grade CSTA Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Cystatin A (Stefin A) (CSTA) (AA 2-97) protein (His tag)Crystallography grade CSTA Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % glycerol
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CSTA (Cystatin A (Stefin A) (CSTA))
- Andere Bezeichnung
- CSTA (CSTA Produkte)
- Synonyme
- AREI Protein, STF1 Protein, STFA Protein, CST Protein, CSTA Protein, ATCYSA Protein, FL3-27 Protein, T20B5.8 Protein, T20B5_8 Protein, cystatin A Protein, cystatin A Protein, cystatin A1 Protein, cystatin-A Protein, CSTA Protein, Csta1 Protein, Csta Protein, CYSA Protein, LOC100725920 Protein
- Hintergrund
- CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies Recombinant human CSTA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 13.1 kDa (118aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_005204
- UniProt
- P01040
- Pathways
- Response to Water Deprivation
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