PDIA3 Protein (AA 25-505) (His tag)

Produktnummer ABIN6387614

Produktdetails

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 25-505

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PDIA3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL

Reinheit: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 0.1 M NaCl, and 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Andere Bezeichnung: PDIA3 (PDIA3 Produkte)

Synonyme: ER60 Protein, ERp57 Protein, ERp60 Protein, ERp61 Protein, GRP57 Protein, GRP58 Protein, HsT17083 Protein, P58 Protein, PI-PLC Protein, grp-58 Protein, 58kDa Protein, Erp Protein, Grp58 Protein, PDI Protein, PDI-Q2 Protein, PLC[a] Protein, Plca Protein, 1 Protein, 25D3-membrane-associated Protein, sb:cb825 Protein, erp57 Protein, grp58 Protein, pdia3 Protein, protein disulfide isomerase family A member 3 Protein, protein disulfide-isomerase A3 Protein, protein disulfide isomerase associated 3 Protein, protein disulfide isomerase family A, member 3 Protein, protein disulfide isomerase family A member 3 S homeolog Protein, PDIA3 Protein, Tsp_10062 Protein, Pdia3 Protein, pdia3 Protein, pdia3.S Protein

Hintergrund: PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 58.5 kDa (518aa), confirmed by MALDI-TOF

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis