RDBP Protein (AA 1-380) (His tag)

Produktnummer ABIN6387428

Produktdetails

Target: RDBP (RD RNA Binding Protein (RDBP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-380

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses RDBP Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.2M NaCl, 50 % glycerol, 5  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: RDBP (RD RNA Binding Protein (RDBP))

Andere Bezeichnung: NELFE (RDBP Produkte)

Synonyme: CG5994 Protein, Dmel\\CG5994 Protein, NELF Protein, NELF-E Protein, Nelf-e Protein, anon-66Da Protein, cg5994 Protein, dNelf-E Protein, D6S45 Protein, RD Protein, RDBP Protein, RDP Protein, Rd Protein, Rdbp Protein, D17H6S45 Protein, Negative elongation factor E Protein, negative elongation factor complex member E Protein, negative elongation factor complex member E, Rdbp Protein, Nelf-E Protein, NELFE Protein, Nelfe Protein

Hintergrund: NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins, however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Molekulargewicht: 45.6kDa (403aa) confirmed by MALDI-TOF

NCBI Accession: NP_002895

UniProt: P18615