NDUFA5 Protein (AA 1-116) (His tag)

Produktnummer ABIN6387051

Dieses Recombinant NDUFA5-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für NDUFA5 Protein (AA 1-116) (His tag) (ABIN6387051)

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 85 %

Produktdetails

Proteineigenschaft: AA 1-116

Aufreinigungstag / Konjugat: Dieses NDUFA5 Protein ist gelabelt mit His tag.

Sequenz: MAGVLKK TTGLVGLAVC NTPHERLRIL YTKILDVLEE IPKNAAYRKY TEQITNEKLA MVKAEPDVKK LEDQLQGGQL EEVILQAEHE LNLARKMREW KLWEPLVEEP PADQWKWPI

Aufreinigung: > 85% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: NDUFA5 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 5, 13kDa (NDUFA5))

Andere Bezeichnung: NDUFA5

Hintergrund: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, also known as NDuFA5, belongs to the complex I NDuFA5 subunit family. The human NDuFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDuFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. Recombinant human NDuFA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Molekulargewicht: 15.8 kDa (139aa) confirmed by MALDI-TOF

NCBI Accession: NP_004991