PSMB10 Protein (AA 40-273) (His tag)

Produktnummer ABIN7279563

Produktdetails

Target: PSMB10 (Proteasome (Prosome, Macropain) Subunit, beta Type 10 (PSMB10))

Protein-Typ: Recombinant

Proteineigenschaft: AA 40-273

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PSMB10 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: PSMB10, 40-273aa, Human, His tag, E.coli

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl

Lagerung: 4 °C

Antigendetails

Target: PSMB10 (Proteasome (Prosome, Macropain) Subunit, beta Type 10 (PSMB10))

Andere Bezeichnung: PSMB10 (PSMB10 Produkte)

Synonyme: Mecl-1 Protein, Mecl1 Protein, LMP10 Protein, MECL1 Protein, beta2i Protein, proteasome (prosome, macropain) subunit, beta type 10 Protein, proteasome subunit beta 10 Protein, Psmb10 Protein, PSMB10 Protein

Hintergrund: PSMB10 is a member of the proteasome B-type family, also known as the T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Expression of the PSMB10 gene is induced by gamma interferon, and PSMB10 replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. Recombinant human PSMB10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Proteasome subunit beta type-10, LMP10, MECL1. NCBI no.: NP_002792

Molekulargewicht: 26.9 kDa (255aa), confirmed by MALDI-TOF

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA