CD19 Protein (AA 20-291) (His tag)

Produktnummer ABIN6253679

Produktdetails

Target: CD19 (CD19 Molecule (CD19))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 20-291

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD19 Protein ist gelabelt mit His tag.

Sequenz: AA 20-291

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >90 % as determined by reduced SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Zhang, Sun, Wang, Du, Gao, Yuan, Zhao, Yang, Xu, Lei, Duan, Xu, Ma, Wang, Hu, Chen, Wang, Hu, Zhang: "Secretion of human soluble programmed cell death protein 1 by chimeric antigen receptor-modified T cells enhances anti-tumor efficacy." in: Cytotherapy, Vol. 22, Issue 12, pp. 734-743, (2020) (PubMed).

Antigendetails

Target: CD19 (CD19 Molecule (CD19))

Andere Bezeichnung: CD19 (CD19 Produkte)

Synonyme: B4 Protein, CVID3 Protein, AW495831 Protein, CD19 Protein, CD19 molecule Protein, CD19 antigen Protein, CD19 Protein, Cd19 Protein

Hintergrund: B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

Molekulargewicht: 32.0 kDa

NCBI Accession: NP_001761

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung