Arylsulfatase B Protein (ARSB) (AA 81-533) (His tag)
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- Target Alle Arylsulfatase B (ARSB) Proteine anzeigen
- Arylsulfatase B (ARSB)
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 81-533
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses Arylsulfatase B Protein ist gelabelt mit His tag.
- Applikation
- Cell Culture (CC), Activity Assay (AcA)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 80 %
- Biological Activity Comment
- Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family. ARSB hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSB and uPAR was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ARSB with uPAR.
- Top Product
- Discover our top product ARSB Protein
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- Applikationshinweise
- Isoelectric Point: 7.7
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- Arylsulfatase B (ARSB)
- Abstract
- ARSB Produkte
- Synonyme
- ASB Protein, G4S Protein, MPS6 Protein, 1110007C02Rik Protein, AI480648 Protein, As-1 Protein, As-1r Protein, As-1s Protein, As-1t Protein, As1 Protein, As1-r Protein, As1-s Protein, As1-t Protein, Asr-1 Protein, Ast-1 Protein, ARSB Protein, arylsulfatase B Protein, arylsulfatase Protein, arylsulfatase b Protein, ARSB Protein, Arsb Protein, RB348 Protein, LOC5566067 Protein, CpipJ_CPIJ011047 Protein, VDBG_03275 Protein, arsb Protein, LOC5579667 Protein
- Hintergrund
- Alternative Names: ASB, G4S, N-acetylgalactosamine-4-sulfatase
- Molekulargewicht
- 55kDa
- UniProt
- P15848
- Pathways
- Glycosaminoglycan Metabolic Process
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