CYP1A1 Protein (AA 251-521) (His tag,GST tag)

Produktnummer ABIN6239867

Produktdetails

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Biologische Aktivität: Active

Proteineigenschaft: AA 251-521

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CYP1A1 Protein ist gelabelt mit His tag,GST tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His and GST Tag

Reinheit: > 95 %

Biological Activity Comment: Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamily of enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4 (HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CYP1A1 and recombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPA4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CYP1A1 and HSPA4 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CYP1A1 with HSPA4

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Andere Bezeichnung: Cytochrome P450 1A1 (CYP1A1) (CYP1A1 Produkte)

Synonyme: AHH Protein, AHRR Protein, CP11 Protein, CYP1 Protein, P1-450 Protein, P450-C Protein, P450DX Protein, Cyp1a2 Protein, P450-1 Protein, cyp1a1 Protein, CYP1A1 Protein, CYPIA1 Protein, Cyp45c Protein, Cypc45c Protein, P-450MC Protein, wu:fb63b04 Protein, zfCYP1A Protein, zgc:109747 Protein, ahh Protein, ahrr Protein, cp11 Protein, cyp1 Protein, cyp1a Protein, p1-450 Protein, p450-c Protein, p450dx Protein, cytochrome P450 family 1 subfamily A member 1 Protein, cytochrome P450 1A1 Protein, cytochrome P450, family 1, subfamily a, polypeptide 1 Protein, cytochrome P450, family 1, subfamily A, polypeptide 1 Protein, cytochrome P450 family 1 subfamily D polypeptide 1 Protein, cytochrome P4501A1 Protein, polycyclic hydrocarbon-inducible cytochrome P450c Protein, cytochrome P450, family 1, subfamily A Protein, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 Protein, cytochrome P450 family 1 subfamily A member 1 L homeolog Protein, CYP1A1 Protein, CpipJ_CPIJ010542 Protein, Cyp1a1 Protein, cyp1d1 Protein, LOC100328613 Protein, cyp1a Protein, LOC102129994 Protein, cyp1a1.L Protein

Hintergrund: Alternative Names: CYP1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1

Molekulargewicht: 62kDa

UniProt: P00185

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha