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Vitamin D Receptor Protein (VDR) (AA 272-427) (His tag)

VDR Spezies: Human Wirt: Escherichia coli (E. coli) > 90 % CC, AcA Active
Produktnummer ABIN6239849
  • Target Alle Vitamin D Receptor (VDR) Proteine anzeigen
    Vitamin D Receptor (VDR)
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 272-427
    Spezies
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses Vitamin D Receptor Protein ist gelabelt mit His tag.
    Applikation
    Cell Culture (CC), Activity Assay (AcA)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 90 %
    Biological Activity Comment
    Vitamin D Receptor (VDR) is the nuclear hormone receptor for vitamin D3. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. It mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Besides, E1A binding protein p300 (EP300) has been identified as an interactor of VDR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VDR and recombinant human EP300. Briefly, VDR were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C . Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of VDR and EP300 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of VDR with EP300.
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  • Applikationshinweise
    Isoelectric Point: 6.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    100 mMNaHCO3, 500 mMNaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    Vitamin D Receptor (VDR)
    Abstract
    VDR Produkte
    Synonyme
    vdrbeta Protein, vdr0 Protein, LOC100136219 Protein, NR1I1-B Protein, gb:dq017633 Protein, vdr-b Protein, Ci-VDR-b Protein, VDR Protein, LOC100221284 Protein, vdr-A Protein, xVDR Protein, Nr1i1 Protein, vdr Protein, NR1I1 Protein, PPP1R163 Protein, vitamin D (1,25- dihydroxyvitamin D3) receptor Protein, vitamin D receptor Protein, vitamin D3 receptor A Protein, vitamin D receptor b Protein, nuclear receptor VDR-b Protein, vitamin D (1,25- dihydroxyvitamin D3) receptor L homeolog Protein, vitamin D (1,25-dihydroxyvitamin D3) receptor Protein, vitamin D receptor a Protein, vdr Protein, vdr0 Protein, VDR Protein, LOC100136219 Protein, vdrb Protein, vdr-b Protein, vdr.L Protein, Vdr Protein, vdra Protein
    Substanzklasse
    Chemical
    Hintergrund
    Alternative Names: NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor
    Molekulargewicht
    20kDa
    UniProt
    P11473
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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