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NEU1 Protein (AA 141-286) (His tag)

NEU1 Spezies: Human Wirt: Escherichia coli (E. coli) > 90 % AcA, CC Active
Produktnummer ABIN6239835
  • Target Alle NEU1 Proteine anzeigen
    NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 141-286
    Spezies
    • 9
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses NEU1 Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 90 %
    Biological Activity Comment
    NEU (Sialidase-1) is an enzyme that catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (CTSA). Thus a binding ELISA assay was conducted to detect the interaction of recombinant human NEU and recombinant human CTSA. Briefly, NEU were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CTSA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NEU pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NEU and CTSA was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of NEU with CTSA.
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  • Applikationshinweise
    Isoelectric Point: 5.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    NEU1 (Sialidase 1 (Lysosomal Sialidase) (NEU1))
    Andere Bezeichnung
    Neuraminidase (NEU) (NEU1 Produkte)
    Synonyme
    NANH Protein, NEU Protein, SIAL1 Protein, MGC81958 Protein, AA407268 Protein, AA407316 Protein, Aglp Protein, Apl Protein, Bat-7 Protein, Bat7 Protein, G9 Protein, Map-2 Protein, Neu Protein, Neu-1 Protein, neuraminidase 1 Protein, neuraminidase 1 (lysosomal sialidase) L homeolog Protein, NEU1 Protein, neu1.L Protein, neu1 Protein, Neu1 Protein
    Substanzklasse
    Influenza Protein
    Hintergrund
    Alternative Names: NEU1, SIAL1, Sialidase 1, Lysosomal Sialidase, Acetylneuraminyl hydrolase, G9 sialidase, N-acetyl-alpha-neuraminidase 1
    Molekulargewicht
    26kDa
    UniProt
    Q99519
    Pathways
    SARS-CoV-2 Protein Interaktom
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