VEGFC Protein (AA 47-413) (His tag)

Produktnummer ABIN6239783

Produktdetails

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Biologische Aktivität: Active

Proteineigenschaft: AA 47-413

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses VEGFC Protein ist gelabelt mit His tag.

Applikation: Cell Culture (CC), Activity Assay (AcA)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 90 %

Biological Activity Comment: Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339μg/mL The dose-effect curve of VEGFC on ECV304 cells

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 8.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Abstract: VEGFC Produkte

Synonyme: VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein

Hintergrund: Alternative Names: VEGF-C, Flt4-L, VRPL, Vascular Endothelial Growth Factor-Related Protein

Molekulargewicht: 45kDa

UniProt: P49767

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2