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TNFRSF12A Protein (AA 24-126) (His tag,GST tag)

TNFRSF12A Spezies: Human Wirt: Escherichia coli (E. coli) > 80 % AcA, CC Active
Produktnummer ABIN6239776
  • Target Alle TNFRSF12A Proteine anzeigen
    TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 24-126
    Spezies
    • 15
    • 4
    Human
    Quelle
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses TNFRSF12A Protein ist gelabelt mit His tag,GST tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His and GST Tag
    Reinheit
    > 80 %
    Biological Activity Comment
    TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TNFRSF12A with TNFa.
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  • Applikationshinweise
    Isoelectric Point: 6.7
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))
    Abstract
    TNFRSF12A Produkte
    Synonyme
    CD266 Protein, FN14 Protein, TWEAKR Protein, AI255180 Protein, C87282 Protein, Fn14 Protein, HPIP Protein, TWEAK-R Protein, TweakR Protein, TNF receptor superfamily member 12A Protein, tumor necrosis factor receptor superfamily, member 12a Protein, TNFRSF12A Protein, Tnfrsf12a Protein
    Hintergrund
    Alternative Names: CD266, TNFRSF12-A, FN14, TWEAKR, Tumour Necrosis Factor Related Weak Inducer Of Apoptosis, Fibroblast growth factor-inducible immediate-early response protein 14
    Molekulargewicht
    44kDa
    UniProt
    Q9NP84
    Pathways
    Apoptose, Regulation of Cell Size
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