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IL-11 Protein (AA 22-199) (His tag)

IL11 Spezies: Human Wirt: Escherichia coli (E. coli) > 90 % AcA, CC Active
Produktnummer ABIN6239760
  • Target Alle IL-11 (IL11) Proteine anzeigen
    IL-11 (IL11) (Interleukin 11 (IL11))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 22-199
    Spezies
    • 19
    • 11
    • 2
    • 2
    Human
    Quelle
    • 13
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses IL-11 Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 90 %
    Biological Activity Comment
    IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL11 significantly promoted cell proliferation of Jurkat cells.
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  • Applikationshinweise
    Isoelectric Point: 11.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    IL-11 (IL11) (Interleukin 11 (IL11))
    Abstract
    IL11 Produkte
    Synonyme
    IL11 Protein, AGIF Protein, IL-11 Protein, Il-11 Protein, interleukin 11 Protein, IL11 Protein, Il11 Protein
    Hintergrund
    Alternative Names: AGIF, Adipogenesis Inhibitory Factor, Oprelvekin
    Molekulargewicht
    27kDa
    UniProt
    P20809
    Pathways
    JAK-STAT Signalweg, Negative Regulation of Hormone Secretion
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