IL-6 Protein (AA 25-211) (His tag)

Produktnummer ABIN6239753

Produktdetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Biologische Aktivität: Active

Proteineigenschaft: AA 25-211

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-6 Protein ist gelabelt mit His tag.

Applikation: Cell Culture (CC), Activity Assay (AcA)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL6 induces C-reactive protein (CRP) expression through the STAT3 pathway in HepG2 cells. To test this effect, HepG2 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without various concentrations of IL6 for 24h and CRP levels in the cell supernatant were determined by ELISA. CRP levels in the cell supernatant of HepG2 cells increased significantly after stimulated with IL6, the data was shown in Table 1 and Figure 1. Sample Concentration of CRP O.D. value Corrected (cell supernatant of HepG2 cells) (ng/mL) stimulated with IL6 (10ng/mL) 0.514 0.453 1.61 stimulated with IL6 (30ng/mL) 0.396 0.335 1.13 unstimulated 0.128 0.067 0.03 Table 1. CRP levels in the cell supernatant of HepG2 cells regulated by IL6 CRP levels in the cell supernatant of HepG2 cells regulated by IL6.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Abstract: IL6 Produkte

Synonyme: BSF2 Protein, HGF Protein, HSF Protein, IFNB2 Protein, IL-6 Protein, Il-6 Protein, ILg6 Protein, Ifnb2 Protein, il6 Protein, CHIL-6 Protein, interleukin 6 Protein, interleukin-6 Protein, IL6 Protein, Il6 Protein, il-6 Protein, IL-6 Protein

Hintergrund: Alternative Names: MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor

Molekulargewicht: 26-33kDa

UniProt: P08505

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome