FNDC5 Protein (AA 32-143) (His tag)

Produktnummer ABIN6239745

Produktdetails

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Biologische Aktivität: Active

Proteineigenschaft: AA 32-143

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses FNDC5 Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 97 %

Biological Activity Comment: The protein encoded by FNDC5 can be cleaved into Irisin, which is a myokine linked to exercise and lean body mass. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1. .

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.6, containing 5 % trehalose.

Antigendetails

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Andere Bezeichnung: Fibronectin Type III Domain Containing Protein 5 (FNDC5) (FNDC5 Produkte)

Synonyme: FRCP2 Protein, irisin Protein, 1500001L03Rik Protein, AI836596 Protein, C87088 Protein, PeP Protein, Pxp Protein, fndc5 Protein, si:dkey-264p5.1 Protein, zgc:158684 Protein, fibronectin type III domain containing 5 Protein, fibronectin type III domain containing 5b Protein, FNDC5 Protein, Fndc5 Protein, fndc5b Protein

Hintergrund: Alternative Names: FRCP2, Irisin, Fibronectin type III repeat-containing protein 2

Molekulargewicht: 27kDa

UniProt: Q8NAU1

Pathways: Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation