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CD3 epsilon Protein (CD3E) (AA 23-126) (His tag,AVI tag,Biotin)

CD3E Spezies: Human Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Produktnummer ABIN5954965
  • Target Alle CD3 epsilon (CD3E) Proteine anzeigen
    CD3 epsilon (CD3E)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 23-126
    Spezies
    • 24
    • 9
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 22
    • 8
    • 5
    • 5
    • 2
    • 2
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses CD3 epsilon Protein ist gelabelt mit His tag,AVI tag,Biotin.
    Sequenz
    AA 23-126
    Spezifität
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Reinheit
    >90 % as determined by SDS-PAGE.
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product CD3E Protein
  • Kommentare

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handhabung
    Please avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Knowling, Clark, Sjuts, Abdiche: "Direct Comparison of Label-Free Biosensor Binding Kinetics Obtained on the Biacore 8K and the Carterra LSA." in: SLAS discovery : advancing life sciences R & D, Vol. 25, Issue 9, pp. 977-984, (2020) (PubMed).

  • Target
    CD3 epsilon (CD3E)
    Andere Bezeichnung
    CD3 epsilon (CD3E Produkte)
    Synonyme
    cd3e Protein, CD3E Protein, t3e Protein, tcre Protein, AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, T3E Protein, TCRE Protein, CD3e molecule Protein, CD3 antigen, epsilon polypeptide Protein, cd3e Protein, CD3E Protein, Cd3e Protein
    Hintergrund
    CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
    Molekulargewicht
    15.5 kDa
    NCBI Accession
    NP_000724
    Pathways
    T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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