Epidermal Growth Factor (EGF) (Active) Protein

Details zu Produkt Nr. ABIN593405
Proteinname
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Spezies
Human
79
43
24
7
4
1
1
Quelle
Escherichia coli (E. coli)
112
8
5
5
4
4
3
2
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Optionen
Produktmerkmale Molecule Information: Human EGF is a 6.2 kDa recombinant protein
Reinheit ≥98 % by SDS-PAGE
≥98 % by HPLC
Endotoxin-Niveau <0.1 ng/μg
Biological Activity Comment The ED₅₀ of human EGF is determined by the dose-dependent stimulation of BALB/c 3T3 cells is 5.92-10.06 ng/ml.
Proteinname
Hintergrund Human epidermal growth factor (EGF) is also known as HOMG4 and URG, and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk. Recombinant human EGF is a 6.2 kDa protein containing 53 amino acid residues. This recombinant human EGF has an N-terminal His-tag preceding the 53 amino acid sequence (MW = 8.5 kDa)

Alternative Names: Urogastrone, URG, EGF, epidermal growth factor
UniProt Q6QBS2
Pathways NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute the human EGF in H₂O to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Buffer EGF protein is lyophilized with no additives
Handhabung Centrifuge the vial prior to opening.
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
 image for Epidermal Growth Factor (EGF) (Active) protein (ABIN593405) Epidermal Growth Factor (EGF) (Active) protein
Produkt verwendet in: Wang, Qian, Li, Pan, Njunge, Dong, Yang: "Evaluation of emulsion electrospun polycaprolactone/hyaluronan/epidermal growth factor nanofibrous scaffolds for wound healing." in: Journal of biomaterials applications, 2015 (PubMed).

An, Gip, Kim, Wu, Park, McVaugh, Schaffer, Bertozzi, Lebrilla: "Extensive determination of glycan heterogeneity reveals an unusual abundance of high mannose glycans in enriched plasma membranes of human embryonic stem cells." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 4, pp. M111.010660, 2012 (PubMed).