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DDT Protein (AA 21-248) (His tag)
DDT
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
Produktnummer ABIN5855098
Produktdetails
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Target
Alle DDT Proteine anzeigen
DDT
(D-Dopachrome Tautomerase (DDT))
Protein-Typ
Recombinant
Proteineigenschaft
AA 21-248
Spezies
Alle Spezies für DDT Proteine
Maus
Quelle
Alle Expressionssysteme für DDT Proteine
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses DDT Protein ist gelabelt mit His tag.
Applikation
SDS-PAGE (SDS)
Sequenz
MGSSHHHHHH SSGLVPRGSH MGSMPFVELE TNLPASRIPA GLENRLCAAT ATILDKPEDR VSVTIRPGMT LLMNKSTEPC AHLLVSSIGV VGTAEQNRTH SASFFKFLTE ELSLDQDRIV IRFFPLEAWQ IGKKGTVMTF L
Reinheit
> 95 % by SDS - PAGE
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Format
Liquid
Konzentration
1 mg/mL
Buffer
Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Antigendetails
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Target
DDT
(D-Dopachrome Tautomerase (DDT))
Andere Bezeichnung
D-Dopachrome Decarboxylase (DDT Produkte )
Synonyme
ddt-b Protein, zgc:86714 Protein, wu:fb49f11 Protein, C78655 Protein, DDT Protein, ddt Protein, ddt-a Protein, DDCT Protein, D-dopachrome tautomerase L homeolog Protein, D-dopachrome tautomerase Protein, D-dopachrome tautomerase S homeolog Protein, ddt.L Protein, DDT Protein, ddt Protein, Ddt Protein, ddt.S Protein
Hintergrund
Ddt, also known as D-dopachrome decarboxylase, is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihyroxyindole(DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33 % identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity in addition, it may functions a proinflammatory cytokine. Recombinant mouse Ddt, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Molekulargewicht
15.5 kDa (141aa) confirmed by MALDI-TOF
NCBI Accession
NP_034157
UniProt
O35215
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