SPR Protein (AA 1-262) (His tag)
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- Target Alle SPR Proteine anzeigen
- SPR (Sepiapterin Reductase (SPR))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-262
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Spezies
- Maus
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses SPR Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLA AADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNIS SLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDNDMQ QLARETSKDP ELRSKLQKLK SDGALVDCGT SAQKLLGLLQ KDTFQSGAHV DFYDC
- Reinheit
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product SPR Protein
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Sepiapterin Reductase (SPR) (AA 1-261) protein (Strep Tag)
Crystallography grade SPR Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Sepiapterin Reductase (SPR) (AA 1-261) protein (GST tag)SPR Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sepiapterin Reductase (SPR) (AA 164-260) protein (GST tag)SPR Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sepiapterin Reductase (SPR) (AA 1-261) protein (Strep Tag)Crystallography grade SPR Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Sepiapterin Reductase (SPR) protein (Myc-DYKDDDDK Tag)SPR Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.5) containing 1 mM DTT, 10 % glycerol
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- SPR (Sepiapterin Reductase (SPR))
- Andere Bezeichnung
- Spr (SPR Produkte)
- Synonyme
- SDR38C1 Protein, AA409688 Protein, ENSMUSG00000071345 Protein, Gm10328 Protein, CG12117 Protein, Dmel\\CG12117 Protein, SR Protein, sptr Protein, xspr Protein, DDBDRAFT_0188686 Protein, DDBDRAFT_0191275 Protein, DDB_0188686 Protein, DDB_0191275 Protein, zgc:194446 Protein, sepiapterin reductase Protein, Sepiapterin reductase Protein, sepiapterin reductase L homeolog Protein, sepiapterin reductase b Protein, SPR Protein, Spr Protein, Sptr Protein, spr.L Protein, sprE Protein, CpipJ_CPIJ000511 Protein, sr Protein, spr Protein, sprb Protein
- Hintergrund
- Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molekulargewicht
- 30.3kDa (285aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_035597
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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