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VCAM1 Protein (AA 25-698) (His tag)

VCAM1 Spezies: Human Wirt: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
Produktnummer ABIN5854738
  • Target Alle VCAM1 Proteine anzeigen
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 25-698
    Spezies
    • 26
    • 14
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Quelle
    • 21
    • 19
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Baculovirus infected Insect Cells
    Aufreinigungstag / Konjugat
    Dieses VCAM1 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    ADPEFFKIET TPESRYLAQI GDSVSLTCST TGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDP EIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDI GKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWS KKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGD SVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVE LYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMT FIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGF PAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFP SESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLR SLTLDVQGRE NNKDYFSPEH HHHHH
    Reinheit
    > 90 % by SDS - PAGE
    Endotoxin-Niveau
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Biological Activity Comment
    Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates 10ug/ml. This effect is more to 70%.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Bioactivity Validated

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
    Andere Bezeichnung
    VCAM1 (VCAM1 Produkte)
    Synonyme
    CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein
    Hintergrund
    VCAM1, also known as vascular cell adhesion protein 1 isoform, a member of the immunoglobulin superfamily. It is a cell surface protein expressed by activated endothelial cells and certain leukocytes. VCAM1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection and asthmatic responses. During the inflammatory adhesion mechanism, activated integrins that rolling leukocytes and attach them firmly to the vascular endothelium. Recombinant human VCAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molekulargewicht
    75.6kDa (685aa) 70-100KDa (SDS-PAGE under reducing conditions.)
    NCBI Accession
    NP_001069
    UniProt
    P19320
    Pathways
    Carbohydrate Homeostasis
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