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P4HB Protein (AA 20-509) (His tag)

P4HB Spezies: Maus Wirt: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS Active
Produktnummer ABIN5854508
  • Target Alle P4HB Proteine anzeigen
    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 20-509
    Spezies
    • 11
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 15
    • 10
    • 4
    • 2
    • 1
    • 1
    Baculovirus infected Insect Cells
    Aufreinigungstag / Konjugat
    Dieses P4HB Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA AKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKN GDTASPKEYT AGREADDIVN WLKKRTGPAA TTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQ LDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANF EEVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDG AGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH
    Reinheit
    > 95 % by SDS - PAGE
    Endotoxin-Niveau
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Biological Activity Comment
    Specific activity is >120 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.
    Top Product
    Discover our top product P4HB Protein
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Bioactivity Validated

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
    Andere Bezeichnung
    P4HB (P4HB Produkte)
    Synonyme
    DSI Protein, ERBA2L Protein, GIT Protein, P4Hbeta Protein, PDI Protein, PDIA1 Protein, PHDB Protein, PO4DB Protein, PO4HB Protein, PROHB Protein, PDA2 Protein, PDIP Protein, PDIR Protein, ERp59 Protein, Pdia1 Protein, Thbp Protein, p55 Protein, XPDIp Protein, pdi Protein, 1810041F13Rik Protein, AI661267 Protein, Pdip Protein, Pdipl Protein, p58 Protein, prolyl 4-hydroxylase subunit beta Protein, protein disulfide isomerase family A member 2 Protein, prolyl 4-hydroxylase, beta polypeptide Protein, protein disulfide isomerase family A member 2 L homeolog Protein, protein disulfide isomerase associated 2 Protein, prolyl 4-hydroxylase subunit beta L homeolog Protein, protein disulfide-isomerase Protein, P4HB Protein, PDIA2 Protein, P4hb Protein, pdia2.L Protein, Pdia2 Protein, p4hb.L Protein, LOC8281065 Protein
    Hintergrund
    P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molekulargewicht
    56.1kDa (498aa) 50-70KDa (SDS-PAGE under reducing conditions)
    NCBI Accession
    NP_035162
    UniProt
    P09103
    Pathways
    Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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