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CA12 Protein (AA 25-301) (His tag)

CA12 Spezies: Human Wirt: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
Produktnummer ABIN5854284
  • Target Alle CA12 Proteine anzeigen
    CA12 (Carbonic Anhydrase 12 (CA12))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 25-301
    Spezies
    • 13
    • 7
    • 2
    • 2
    • 1
    Human
    Quelle
    • 9
    • 8
    • 2
    • 2
    • 2
    • 2
    Baculovirus infected Insect Cells
    Aufreinigungstag / Konjugat
    Dieses CA12 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLSHHH HHH
    Reinheit
    > 90 % by SDS - PAGE
    Endotoxin-Niveau
    < 1.0 EU per 1 microgram of protein (determined by LAL method)
    Biological Activity Comment
    Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
    Top Product
    Discover our top product CA12 Protein
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Bioactivity Validated

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Liquid. In phosphate buffered saline (  pH 7.4) containing 10 % glycerol.
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
  • Target
    CA12 (Carbonic Anhydrase 12 (CA12))
    Andere Bezeichnung
    CA12 (CA12 Produkte)
    Synonyme
    CAXII Protein, HsT18816 Protein, CAR12 Protein, 2310047E01Rik Protein, AI314958 Protein, Ca12 Protein, ca12 Protein, carbonic anhydrase 12 Protein, carbonic anhydrase XII L homeolog Protein, CA12 Protein, Car12 Protein, ca12.L Protein
    Hintergrund
    CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
    Molekulargewicht
    31.94kDa (283aa) (28-40kDa in SDS-PAGE under reducing conditions.)
    NCBI Accession
    NP_001209
    UniProt
    O43570
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