CST5 Protein (AA 26-142) (His tag)

Produktnummer ABIN5854281

Produktdetails

Target: CST5 (Cystatin D (CST5))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 26-142

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses CST5 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: QSRTLAGGIH ATDLNDKSVQ RALDFAISEY NKVINKDEYY SRPLQVMAAY QQIVGGVNYY FNVKFGRTTC TKSQPNLDNC PFNDQPKLKE EEFCSFQINE VPWEDKISIL NYKCRKVHHH HHH

Reinheit: > 90 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: The IC50 value is < 2.0nM. The inhibitory function of Cystatin 5 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In phosphate buffered saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CST5 (Cystatin D (CST5))

Andere Bezeichnung: CST5 (CST5 Produkte)

Synonyme: Cst10 Protein, Cystatin-5 Protein, cystatin D Protein, CST5 Protein, Cst5 Protein

Hintergrund: CST5, also known as Cystatin-D, is a novel member of the cystatin superfamily. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. Two alleles of the cystatin D gene (CST5), encoding protein variants with either Cys or Arg as residue 26 in their 122-residue polypeptide chains, are present in the population. Recombinant human CST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 14.36kDa (123aa) (13.5-18kDa in SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001891

UniProt: P28325