PPM1F Protein (AA 1-454) (His tag)

Produktnummer ABIN5853181

Produktdetails

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-454

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PPM1F Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1  mM DTT.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))

Andere Bezeichnung: PPM1F (PPM1F Produkte)

Synonyme: 1110021B16Rik Protein, 4933427B07Rik Protein, CaMKPase Protein, Popx2 Protein, mKIAA0015 Protein, CAMKP Protein, FEM-2 Protein, POPX2 Protein, hFEM-2 Protein, protein phosphatase, Mg2+/Mn2+ dependent 1F Protein, protein phosphatase 1F (PP2C domain containing) Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1F Protein, PPM1F Protein, Ppm1f Protein

Hintergrund: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 52kDa (477aa)

NCBI Accession: NP_055449

UniProt: P49593

Pathways: Positive Regulation of Endopeptidase Activity