Crystallography grade
COPE
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS, Crys
COPE Protein, DDBDRAFT_0189670 Protein, DDBDRAFT_0233799 Protein, DDB_0189670 Protein, DDB_0233799 Protein, DKFZp469K1716 Protein, MGC80063 Protein, MGC130714 Protein, epsilon-COP Protein, 1110005D17Rik Protein, Cope1 Protein, coatomer protein complex subunit epsilon Protein, coatomer protein complex epsilon subunit Protein, coatomer protein complex subunit epsilon S homeolog Protein, coatomer protein complex, subunit epsilon Protein, COPE Protein, copE Protein, cope.S Protein, cope Protein, Cope Protein
Hintergrund
COPE is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant human COPE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.