RTCD1 Protein (AA 1-366) (His tag)
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- Target Alle RTCD1 Proteine anzeigen
- RTCD1 (RNA terminal Phosphate Cyclase Domain 1 (RTCD1))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-366
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses RTCD1 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSMAGPRVE VDGSIMEGGG QILRVSTALS CLLGLPLRVQ KIRAGRSTPG LRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA AKDTYIIECQ GIGMTNPNL
- Reinheit
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product RTCD1 Protein
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RNA terminal Phosphate Cyclase Domain 1 (RTCD1) (AA 1-366) protein (His tag)
RTCD1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
RNA terminal Phosphate Cyclase Domain 1 (RTCD1) (AA 1-366) protein (GST tag)RTCD1 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
RNA terminal Phosphate Cyclase Domain 1 (RTCD1) (AA 1-366) protein (Strep Tag)Crystallography grade RTCD1 Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
RNA terminal Phosphate Cyclase Domain 1 (RTCD1) (AA 1-366) protein (Strep Tag)Crystallography grade RTCD1 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RTCD1 (RNA terminal Phosphate Cyclase Domain 1 (RTCD1))
- Andere Bezeichnung
- RTCA (RTCD1 Produkte)
- Synonyme
- Dmel\\CG4061 Protein, EG:22E5.3 Protein, RTC1_DROME Protein, DKFZp469J2025 Protein, cb158 Protein, rtcd1 Protein, sb:cb158 Protein, wu:fc62c03 Protein, zgc:56339 Protein, RTCD1 Protein, RPC Protein, RTC1 Protein, 2310009A18Rik Protein, AI450277 Protein, Rtcd1 Protein, RNA 3'-terminal phosphate cyclase Protein, Rtca Protein, RTCA Protein, rtca Protein, rtca.L Protein
- Hintergrund
- RTCA catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP, (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A, (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. Recombinant human RTCA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 41.7 kDa (389aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003720
- UniProt
- O00442
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