MEP1A Protein (AA 22-601) (His tag)
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- Target Alle MEP1A Proteine anzeigen
- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 22-601
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses MEP1A Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK
- Reinheit
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
- Top Product
- Discover our top product MEP1A Protein
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Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (Active) protein (His tag)
MEP1A Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 66-712) protein (His tag)Crystallography grade MEP1A Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 65-713) protein (His tag)Crystallography grade MEP1A Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 66-746) protein (rho-1D4 tag)Crystallography grade MEP1A Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 65-747) protein (rho-1D4 tag)Crystallography grade MEP1A Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer( pH 8.0) containing 10 % glycerol, 1 mM DTT
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
- Andere Bezeichnung
- MEP1A (MEP1A Produkte)
- Synonyme
- AI098089 Protein, AW107200 Protein, Mep-1 Protein, Mep-1a Protein, Mep1 Protein, PPHA Protein, meprin A subunit alpha Protein, meprin 1 alpha Protein, Mep1a Protein, MEP1A Protein
- Hintergrund
- PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 30.9 kDa (274aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002620
- UniProt
- P18669
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