MDH2 Protein (AA 25-338) (His tag)

Produktnummer ABIN5778725

Produktdetails

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 25-338

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MDH2 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK

Reinheit: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In Phosphate buffer saline (  pH 7.4) containing 20 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Andere Bezeichnung: Mdh2 (MDH2 Produkte)

Synonyme: Mdh2b Protein, m-mdh Protein, mdh2 Protein, mor1 Protein, MDH-2 Protein, Mdh Protein, wu:fj48c08 Protein, wu:fj55d06 Protein, zgc:64133 Protein, M-MDH Protein, MDH Protein, MGC:3559 Protein, MOR1 Protein, MMDH Protein, Mdh-2 Protein, Mor-1 Protein, Mor1 Protein, malate dehydrogenase 2 S homeolog Protein, malate dehydrogenase 2 Protein, Malate dehydrogenase 2 Protein, malate dehydrogenase Protein, malate dehydrogenase 2, NAD (mitochondrial) Protein, mdh2.S Protein, MDH2 Protein, Mdh2 Protein, MDH4 Protein, mdh2 Protein

Hintergrund: Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 35.4 kDa (335aa) confirmed by MALDI-TOF

NCBI Accession: NP_032643

UniProt: P08249