GLO1 Protein (AA 1-184) (His tag)

Produktnummer ABIN6387797

Produktdetails

Target: GLO1 (Glyoxalase I (GLO1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-184

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses GLO1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH

Reinheit: > 95 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GLO1 (Glyoxalase I (GLO1))

Andere Bezeichnung: Glo1 (GLO1 Produkte)

Synonyme: GLOD1 Protein, GLYI Protein, 0610009E22Rik Protein, 1110008E19Rik Protein, 2510049H23Rik Protein, AW550643 Protein, GLY1 Protein, Glo-1 Protein, Glo-1r Protein, Glo-1s Protein, Glo1-r Protein, Glo1-s Protein, Qglo Protein, cb554 Protein, zgc:66035 Protein, wu:fb82g09 Protein, glod1 Protein, glyi Protein, glo1 Protein, GLO1 Protein, ATGLX1 Protein, F12F1.32 Protein, F12F1_32 Protein, GLYOXALASE I Protein, glyoxalase I homolog Protein, trypanothione-dependent glyoxalase I Protein, glyoxalase I Protein, glyoxalase 1 Protein, glyoxalase 1 L homeolog Protein, glyoxalase 1 S homeolog Protein, lactoylglutathione lyase Protein, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein Protein, GLO1 Protein, Glo1 Protein, glo1 Protein, glo1.L Protein, glo1.S Protein, GST3 Protein, GLX1 Protein, STY1687 Protein, Bcen_2094 Protein, gloA Protein

Hintergrund: Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 21.8kDa (192aa) 28-40kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_079650

UniProt: Q9CPU0