CST3 Protein (AA 21-140) (His tag)

Produktnummer ABIN5777481

Produktdetails

Target: CST3 (Cystatin C (CST3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 21-140

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses CST3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: ATPKQGPRML GAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNL TDCPFHDQPH LMRKALCSFQ IYSVPWKGTH SLTKFSCKNA HHHHHH

Reinheit: > 95 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CST3 (Cystatin C (CST3))

Andere Bezeichnung: Cystatin-C (CST3 Produkte)

Synonyme: armd11 Protein, fb51d07 Protein, wu:fb24g06 Protein, wu:fb51d07 Protein, wu:fc55f03 Protein, zgc:136227 Protein, ARMD11 Protein, CysC Protein, CYSC Protein, cystatin C Protein, cystatin C L homeolog Protein, cystatin C (amyloid angiopathy and cerebral hemorrhage) Protein, cystatin 3 Protein, CST3 Protein, cst3.L Protein, cst3 Protein, cystatin 3 Protein, Cst3 Protein

Hintergrund: CST3, also known as Cystatin-C is a member of family 2 of the cystatin superfamily. This protein is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy. Recombinant Mouse CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 14.2kDa (126aa) confirmed by MALDI-TOF 13.5-18KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_034106

UniProt: P21460