BTD Protein (AA 44-545) (His tag)
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- Target Alle BTD Proteine anzeigen
- BTD (Biotinidase (BTD))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 44-545
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Spezies
- Human
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Quelle
- Baculovirus infected Insect Cells
- Aufreinigungstag / Konjugat
- Dieses BTD Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH
- Reinheit
- > 85 % by SDS - PAGE
- Endotoxin-Niveau
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
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Biotinidase (BTD) (AA 44-545) protein (His tag)BTD Spezies: Human Wirt: Baculovirus infected Insect Cells Recombinant > 85 % by SDS - PAGE SDS
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- BTD (Biotinidase (BTD))
- Andere Bezeichnung
- BTD (BTD Produkte)
- Synonyme
- BTD Protein, CG3599 Protein, CT12113 Protein, Dmel\\CG3599 Protein, zgc:153333 Protein, Biotinase Protein, Biotinidase Protein, biotinidase Protein, Btnd Protein, btd Protein, BTD Protein, Btd Protein
- Hintergrund
- BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molekulargewicht
- 57.8kDa (510aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_001268652
- UniProt
- P43251
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