POLR3A Protein (AA 392-632, partial) (His-SUMO Tag)

Produktnummer ABIN5710123

Produktdetails

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 392-632, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses POLR3A Protein ist gelabelt mit His-SUMO Tag.

Applikation: SDS-PAGE (SDS)

Sequenz: FPEKVNKANI NFLRKLVQNG PEVHPGANFI QQRHTQMKRF LKYGNREKMA QELKYGDIVE RHLIDGDVVL FNRQPSLHKL SIMAHLARVK PHRTFRFNEC VCTPYNADFD GDEMNLHLPQ TEEAKAEALV LMGTKANLVT PRNGEPLIAA IQDFLTGAYL LTLKDTFFDR AKACQIIASI LVGKDEKIKV RLPPPTILKP VTLWTGKQIF SVILRPSDDN PVRANLRTKG KQYCGKGEDL C

Aufreinigung: SDS-PAGE

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Lagerung: -80 °C,4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Antigendetails

Target: POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))

Andere Bezeichnung: RPC1 (POLR3A Produkte)

Synonyme: 9330175N20Rik Protein, BC053071 Protein, RPC1 Protein, RPC155 Protein, RGD1305574 Protein, ADDH Protein, HLD7 Protein, hRPC155 Protein, polymerase (RNA) III (DNA directed) polypeptide A Protein, RNA polymerase III subunit A Protein, Polr3a Protein, POLR3A Protein

Hintergrund: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA tplate strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix anates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition . Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as tplate for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Molekulargewicht: 43.34 kDa

UniProt: O14802