SIRT3 Protein (AA 127-303) (His tag)

Produktnummer ABIN5665903

Produktdetails

Target: SIRT3 (Sirtuin 3 (SIRT3))

Biologische Aktivität: Active

Proteineigenschaft: AA 127-303

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SIRT3 Protein ist gelabelt mit His tag.

Applikation: Cell Culture (CC), Activity Assay (AcA)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, X-Ray Repair Cross Complementing 6 (XRCC6) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant Rat SIRT3 and recombinant Rat XRCC6. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to XRCC6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and XRCC6 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SIRT3 with XRCC6.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: SIRT3 (Sirtuin 3 (SIRT3))

Abstract: SIRT3 Produkte

Synonyme: zgc:158774 Protein, sirtuin Protein, SIR2L3 Protein, 2310003L23Rik Protein, AI848213 Protein, Sir2l3 Protein, sirtuin 3 Protein, sirt3 Protein, SIRT3 Protein, Sirt3 Protein

Hintergrund: Alternative Names: SIR2L3, Silent Mating Type Information Regulation 2 Homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, SIR2-like protein 3, Regulatory protein SIR2 homolog 3

Molekulargewicht: 22kDa

UniProt: C6ZII9