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SEMA3A Protein (AA 31-141) (His tag)

SEMA3A Spezies: Human Wirt: Escherichia coli (E. coli) > 90 % AcA, CC Active
Produktnummer ABIN5665901
  • Target Alle SEMA3A Proteine anzeigen
    SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 31-141
    Spezies
    • 12
    • 4
    • 3
    Human
    Quelle
    • 7
    • 4
    • 4
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses SEMA3A Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 90 %
    Biological Activity Comment
    The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of SEMA3A with NRP1.
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    Discover our top product SEMA3A Protein
  • Applikationshinweise
    Isoelectric Point: 8.9
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))
    Andere Bezeichnung
    Semaphorin 3A (SEMA3A) (SEMA3A Produkte)
    Synonyme
    COLL1 Protein, HH16 Protein, Hsema-I Protein, Hsema-III Protein, SEMA1 Protein, SEMAD Protein, SEMAIII Protein, SEMAL Protein, SemD Protein, coll-1 Protein, Semad Protein, COLL-1 Protein, SEMA3A Protein, Dsema3a Protein, Sema3A Protein, sema1a Protein, sema3a1 Protein, semaZ1a Protein, sema1b Protein, sema3a2 Protein, semaZ1b Protein, semaphorin 3A Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A Protein, Sema-3a Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa Protein, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab Protein, SEMA3A Protein, Sema3a Protein, Sema-3a Protein, sema3aa Protein, sema3ab Protein
    Hintergrund
    Alternative Names: SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
    Molekulargewicht
    16kDa
    UniProt
    Q14563
    Pathways
    Regulation of Cell Size
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