PTH Protein (AA 32-115) (MBP tag,His tag)

Produktnummer ABIN5665891

Produktdetails

Target: PTH (Parathyroid Hormone (PTH))

Biologische Aktivität: Active

Proteineigenschaft: AA 32-115

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PTH Protein ist gelabelt mit MBP tag,His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: Two N-terminal Tags, His-tag and MBP-tag

Reinheit: > 90 %

Biological Activity Comment: PTH (Parathyroid hormone) is a hormone secreted by the parathyroid glands that is important in bone remodeling. As reported, osteoblast-like cell lines, such as ROS 17/2.8, UMR106, SaOS, U2OS, MG63, that exhibit PTHR1, respond with increased proliferation to PTH. Rat PTH shares similarities with human PTH in amino acid sequence with the identity of 71.3%. Thus, a proliferation assay of rat recombinant PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of U2OS cells after incubation with PTH for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant PTH for 48h. The result was shown in Figure 2. It was obvious that PTH increased cell viability of U2OS cells.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: PTH (Parathyroid Hormone (PTH))

Abstract: PTH Produkte

Synonyme: PTH1 Protein, Pthp Protein, PTH-(1-84) Protein, Pth1 Protein, Pthr1 Protein, PTH Protein, parathyroid hormone Protein, parathyroid hormone S homeolog Protein, PTH Protein, Pth Protein, pth.S Protein

Substanzklasse: Hormone

Hintergrund: Alternative Names: iPTH, Intact Parathyroid Hormone, Parathormone, Parathyrin

Molekulargewicht: 59kDa

UniProt: P04089

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process