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MFAP2 Protein (AA 6-162) (His tag)

MFAP2 Spezies: Human Wirt: Escherichia coli (E. coli) > 95 % AcA, CC Active
Produktnummer ABIN5665879
  • Target Alle MFAP2 Proteine anzeigen
    MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 6-162
    Spezies
    • 8
    • 2
    • 1
    • 1
    Human
    Quelle
    • 5
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses MFAP2 Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 95 %
    Biological Activity Comment
    Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of MFAP2 with FBN1.
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  • Applikationshinweise
    Isoelectric Point: 5.5
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))
    Abstract
    MFAP2 Produkte
    Synonyme
    MAGP Protein, MAGP-1 Protein, MAGP1 Protein, AI893631 Protein, Magp Protein, Magp1 Protein, microfibril associated protein 2 Protein, microfibrillar-associated protein 2 Protein, MFAP2 Protein, Mfap2 Protein
    Hintergrund
    Alternative Names: MAGP1, Microfibril-associated glycoprotein 1
    Molekulargewicht
    38kDa
    UniProt
    P55001
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