MFAP2 Protein (AA 6-162) (His tag)
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- Target Alle MFAP2 Proteine anzeigen
- MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 6-162
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses MFAP2 Protein ist gelabelt mit His tag.
- Applikation
- Activity Assay (AcA), Cell Culture (CC)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 95 %
- Biological Activity Comment
- Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of MFAP2 with FBN1.
- Top Product
- Discover our top product MFAP2 Protein
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- Applikationshinweise
- Isoelectric Point: 5.5
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- MFAP2 (Microfibrillar Associated Protein 2 (MFAP2))
- Abstract
- MFAP2 Produkte
- Synonyme
- MAGP Protein, MAGP-1 Protein, MAGP1 Protein, AI893631 Protein, Magp Protein, Magp1 Protein, microfibril associated protein 2 Protein, microfibrillar-associated protein 2 Protein, MFAP2 Protein, Mfap2 Protein
- Hintergrund
- Alternative Names: MAGP1, Microfibril-associated glycoprotein 1
- Molekulargewicht
- 38kDa
- UniProt
- P55001
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