MBL2 Protein (AA 130-248) (His tag,GST tag)

Produktnummer ABIN5665875

Produktdetails

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Biologische Aktivität: Active

Proteineigenschaft: AA 130-248

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MBL2 Protein ist gelabelt mit His tag,GST tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His and GST Tag

Reinheit: > 97 %

Biological Activity Comment: MBL2 (Mannose-binding protein C) is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different microorganisms, therefore results in activation of the lectin pathway of the complement system. It has been proven that MASP-2 (Mannan-binding lectin serine protease 2) forms complexes with the pattern recognition molecules MBL2, triggers the activation of the complement system. Thus, a functional binding ELISA assay was constructed to detect the association of rhMBL2 with MASP2. Briefly, rhMBL2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MASP2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MBL2 mAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MBL2 with MASP2 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of MBL2 with MASP2.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Andere Bezeichnung: Mannose Binding Lectin (MBL) (MBL2 Produkte)

Synonyme: COLEC1 Protein, HSMBPC Protein, MBL Protein, MBL2D Protein, MBP Protein, MBP-C Protein, MBP1 Protein, MBPD Protein, pMBP-27 Protein, mbl2 Protein, MBL1 Protein, cMBl Protein, collectin Protein, Ab2-001 Protein, Ab2-011 Protein, L-MBP Protein, MBL-C Protein, COLEC2 Protein, mbl Protein, etID42583.2 Protein, fb68b07 Protein, hbl3 Protein, wu:fb68b07 Protein, zgc:109836 Protein, mannose binding lectin 2 Protein, Mannose-binding protein C Protein, mannose-binding lectin (protein C) 2, soluble Protein, mannose-binding lectin (protein C) 2 Protein, mannose-binding lectin family member 3, pseudogene Protein, MBL2 Protein, mbl2 Protein, Mbl2 Protein, MBL3P Protein

Hintergrund: Alternative Names: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

Molekulargewicht: 43kDa

UniProt: P11226

Pathways: Komplementsystem, Positive Regulation of Immune Effector Process