Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

IL-24 Protein (AA 51-206) (His tag)

IL24 Spezies: Human Wirt: Escherichia coli (E. coli) > 90 % AcA, CC Active
Produktnummer ABIN5665855
  • Target Alle IL-24 (IL24) Proteine anzeigen
    IL-24 (IL24) (Interleukin 24 (IL24))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 51-206
    Spezies
    • 13
    • 3
    • 3
    Human
    Quelle
    • 8
    • 4
    • 3
    • 2
    • 2
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses IL-24 Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 90 %
    Biological Activity Comment
    IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.
  • Applikationshinweise
    Isoelectric Point: 8.6
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    IL-24 (IL24) (Interleukin 24 (IL24))
    Abstract
    IL24 Produkte
    Synonyme
    C49A Protein, FISP Protein, IL10B Protein, MDA7 Protein, MOB5 Protein, ST16 Protein, Mda7 Protein, St16 Protein, Mda-7 Protein, IL24 Protein, interleukin 24 Protein, IL24 Protein, Il24 Protein
    Hintergrund
    Alternative Names: C49A, FISP, IL10B, MDA7, Mob-5, ST16, Mda-7, Melanoma Differentiation Association Protein 7, Suppression Of Tumorigenicity 16
    Molekulargewicht
    18kDa
    UniProt
    Q13007
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagie
Sie sind hier:
Kundenservice