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IL-20 Protein (AA 25-176) (His tag)

IL20 Spezies: Human Wirt: Escherichia coli (E. coli) > 90 % AcA, CC Active
Produktnummer ABIN5665854
  • Target Alle IL-20 (IL20) Proteine anzeigen
    IL-20 (IL20) (Interleukin 20 (IL20))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 25-176
    Spezies
    • 12
    • 6
    • 3
    • 1
    Human
    Quelle
    • 12
    • 5
    • 2
    • 2
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses IL-20 Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 90 %
    Biological Activity Comment
    IL20 (Interleukin-20) is a cytokine structurally related to interleukin 10, which is produced by activated keratinocytes and monocytes. It is accepted that IL20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. Thus, proliferation assay of IL20 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL20. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of ECV-304 cells after incubation with IIL20 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL20 for 48h. The result was shown in Figure 2. It was obvious that human IL20 significantly decreased cell viability of ECV-304 cells.
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  • Applikationshinweise
    Isoelectric Point: 8.9
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    IL-20 (IL20) (Interleukin 20 (IL20))
    Abstract
    IL20 Produkte
    Synonyme
    IL-20 Protein, IL10D Protein, ZCYTO10 Protein, Zcyto10 Protein, IL20 Protein, interleukin 20 Protein, IL20 Protein, Il20 Protein
    Hintergrund
    Alternative Names: IL10D, IL10-D, ZCYTO10, Cytokine Zcyto10
    Molekulargewicht
    21kDa
    UniProt
    Q9NYY1
    Pathways
    Protein targeting to Nucleus
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