GZMM Protein (AA 26-257) (His tag)

Produktnummer ABIN5665807

Produktdetails

Target: GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))

Biologische Aktivität: Active

Proteineigenschaft: AA 26-257

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GZMM Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 90 %

Biological Activity Comment: GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2x107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin. Normal Heated 0 4 4 GZMM (μM) Ezrin Actin Figure 1. GZMM cleaves Ezrin

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 10.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))

Andere Bezeichnung: Granzyme M (GZMM) (GZMM Produkte)

Synonyme: LMET1 Protein, MET1 Protein, Lmet1 Protein, MMET-1 Protein, granzyme M Protein, granzyme M (lymphocyte met-ase 1) Protein, LOC100136381 Protein, GZMM Protein, Gzmm Protein

Hintergrund: Alternative Names: GZM-M, LMET1, MET1, Hu-Met-1, Met-ase, Lymphocyte Met Ase 1, Met-1 serine protease, Natural killer cell granular protease

Molekulargewicht: 26kDa

UniProt: P51124