nectin-3 Protein (partial) (Fc Tag)

Produktnummer ABIN5608453

Produktdetails

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: partial

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses nectin-3 Protein ist gelabelt mit Fc Tag.

Applikation: Western Blotting (WB), ELISA

Sequenz: Gly 58 - Asp 400

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Endotoxin level is less than 1.0 EU per ug by the LAL method.

Biological Activity Comment:

Measured by its binding ability in a functional ELISA. Immobilized Human CD113, Fc Tag at 10 μg/mL (100 uL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 0.3-5 μg/mL (QC tested).

Anwendungsinformationen

Applikationshinweise: This recombinant protein can be used for E, WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, pH 7.5

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.

Antigendetails

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Andere Bezeichnung: Nectin-3 (NECTIN3 Produkte)

Synonyme: PVRL3 Protein, CD113 Protein, CDW113 Protein, NECTIN-3 Protein, PPR3 Protein, PRR3 Protein, PVRR3 Protein, 2610301B19Rik Protein, 3000002N23Rik Protein, 4921513D19Rik Protein, AA407785 Protein, AU016832 Protein, AW538082 Protein, nectin cell adhesion molecule 3 Protein, poliovirus receptor-related 3 Protein, Nectin3 Protein, NECTIN3 Protein, PVRL3 Protein, nectin3 Protein

Hintergrund: Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6, MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.

Molekulargewicht: 64.3 kDa

Gen-ID: 25945

NCBI Accession: NP_056295

UniProt: Q9NQS3

Pathways: Cell-Cell Junction Organization